Part:BBa_K4422004
Xa Factor Cleavage Site (Pichia pastoris codon optimized)
Protein expression and purification in recombinant DNA systems is a powerful and widely used technique. There is often a need to express the protein of interest as a fusion protein or chimeric protein. It is often necessary to remove the fusion partner before the recombinant protein of interest can be used for further studies. This deletion process involves the insertion of a unique amino acid sequence that is susceptible to cleavage by a highly specific protease. Thrombin and factor Xa are the most commonly used proteases for this application.
Jenny, R. J., Mann, K. G., & Lundblad, R. L. (2003). A critical review of the methods for cleavage of fusion proteins with thrombin and factor Xa. Protein expression and purification, 31(1), 1–11. https://doi.org/10.1016/s1046-5928(03)00168-2
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
None |